![]() ![]() faecium, which include urinary tract infections (UTIs), bloodstream infections, and endocarditis, among others, represent a major therapeutic challenge due to the typical resistance of this species to multiple antibiotics ( 2). faecium to cause infection in the urinary tract.Įnterococcus faecium, a common human commensal, is currently one of the more problematic causes of hospital-associated (HA) infections in the United States, accounting for approximately 38% of clinical enterococcal isolates ( 1). Our results indicate that EmpA and EmpB, but not EmpC, contribute to biofilm and adherence to ECM proteins however, all the Emp pilins are important for E. Furthermore, we showed that each deletion mutant was significantly attenuated in comparison to the isogenic parental strain, TX82, in a mixed-inoculum UTI model ( P < 0.001 to 0.048), that reconstitution of empA restored virulence in the UTI model, and that deletion of empA also resulted in attenuation in an infective endocarditis model ( P = 0.0088). Significant reductions in adherence to fibrinogen and collagen type I were observed with deletion of empA and empB, while deletion of empC had no adherence defect. Deletion of empB also caused a reduction in biofilm, while EmpC was found to be dispensable. We identified EmpA as the tip of the pili and found that deletion of empA reduced biofilm formation to the same level as deletion of the empABC operon, a phenotype that was restored by reconstituting in situ the empA gene. Here, we studied the contributions of the individual pilus subunits EmpA, EmpB, and EmpC to pilus architecture, biofilm formation, adherence to extracellular matrix (ECM) proteins, and infection. faecium TX82 was attenuated in biofilm formation and in a UTI model. We previously demonstrated that a nonpiliated Δ empABC:: cat derivative of E. ![]() Pili have been shown to play a role in the pathogenesis of Gram-positive bacteria, including E. faecium to cause infection in the urinary tract.Įnterococcus faecium is an important cause of hospital-associated infections, including urinary tract infections (UTIs), bacteremia, and infective endocarditis. ![]() Furthermore, we showed that each deletion mutant was significantly attenuated in comparison to the isogenic parental strain, TX82, in a mixed-inoculum UTI model (P < 0.001 to 0.048), that reconstitution of empA restored virulence in the UTI model, and that deletion of empA also resulted in attenuation in an infective endocarditis model (P = 0.0088). ![]() faecium We previously demonstrated that a nonpiliated ΔempABC::cat derivative of E. Enterococcus faecium is an important cause of hospital-associated infections, including urinary tract infections (UTIs), bacteremia, and infective endocarditis. ![]()
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